PAD DEMINERALIZATION TECHNOLOGY

PRECISION OF DEMINERALIZATION

WHAT IT DOES

PAD is a proprietary technology that precisely manages the demineralization process to consistently target ideal residual calcium levels for optimal osteoinductive potential.

WHY IT'S NEEDED 

Demineralization, or the reduction of mineral content of bone allografts, mimics the body's natural bone remodeling process and exposes native growth factors embedded within the bone matrix (such as BMPs, among others). These 'osteoinductive' growth factors are known to provide signals that help naturally induce the formation of new bone.

WHY IT'S DIFFERENT

HOW IT WORKS

Through a series of computer-controlled steps that are tailored to each graft, demineralizing acid is delivered within a closed-vessel system, then drained and neutralized with buffer until the desired residual calcium level is achieved.

Residual calcium levels are then verified as part of our stringent quality assurance process. 

Peer-Reviewed Publications

Zhang M, Powers RM Jr, Wolfinbarger L Jr. Effect(s) of the demineralization process on the osteoinductivity of demineralized bone matrix. J Periodontol. 1997 Nov;68(11):1085-92. doi: 10.1902/jop.1997.68.11.1085

Turonis JW, McPherson JC 3rd, Cuenin MF, Hokett SD, Peacock ME, Sharawy M. The effect of residual calcium in decalcified freeze-dried bone allograft in a critical-sized defect in the Rattus norvegicus calvarium. J Oral Implantol. 2006;32(2):55-62. doi: 10.1563/780.1

Miron RJ, Zhang Q, Sculean A, Buser D, Pippenger BE, Dard M, Shirakata Y, Chandad F, Zhang Y. Osteoinductive potential of 4 commonly employed bone grafts. Clin Oral Investig. 2016 Nov;20(8):2259-2265. doi: 10.1007/s00784-016-1724-4

Wei L, Miron RJ, Shi B, Zhang Y. Osteoinductive and Osteopromotive Variability among Different Demineralized Bone Allografts. Clin Implant Dent Relat Res. 2015 Jun;17(3):533-42. doi: 10.1111/cid.12118

McLean JB, Carter N, Sohoni P, Moore MA. Cell attachment and osteoinductive properties of tissue engineered, demineralized bone fibers for bone void filling applications. Clinical Implementation of Bone Regeneration and Maintenance, IntechOpen. 2021. doi:10.5772/intechopen.88290

Herold RW, Pashley DH, Camuenin MF, Niagro F, Hokett SD, Peacock ME, Mailhot J, Borke J. The effects of varying degrees of allograft decalcification on cultured porcine osteoclast cells. J Periodontol. 2002 Feb;73(2):213-9. doi: 10.1902/jop.2002.73.2.213